3IA3
A cis-proline in alpha-hemoglobin stabilizing Protein directs the structural reorganization of alpha-hemoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 65.230, 65.230, 439.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.250 - 3.200 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 8764 | |
Completeness [%] | 85.0 | 51.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M MES, pH 6.5, 4% acetonitrile and 14.5% (w/v) PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |