3I6X
Crystal structure of the calponin homology domain of IQGAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9397 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.432, 66.421, 83.014 |
| Unit cell angles | 99.55, 104.07, 108.69 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| Rwork | 0.234 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p2x |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 36983 | |
| <I/σ(I)> | 17.47 | 2.7 |
| Completeness [%] | 98.5 | 96.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M-0.3M Ammonium acetate, 20 % PEG 3350, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
