3I6W
Structure and Activation Mechanism of the CHK2 DNA-Damage Checkpoint Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Detector technology | CCD |
Collection date | 2006-01-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 76.200, 114.700, 123.000 |
Unit cell angles | 84.10, 81.20, 80.70 |
Refinement procedure
Resolution | 30.000 - 3.250 |
R-factor | 0.252 |
Rwork | 0.251 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i6u |
RMSD bond length | 0.009 |
RMSD bond angle | 1.138 |
Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.250 |
Number of reflections | 60983 |
Completeness [%] | 96.2 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1:1 ratio mix of protein solution (~12 mg/ml protein in 20 mM Tris-HCl, 150 mM NaCl, 10 mM dithiothreitol (DTT), 3% (v/v) glycerol, pH 8.0 and well bufffer (100 mM NaHepes, 300 mM Ammonium Tartrate, 22% PEG 3350, pH 7.0). Crystals were flash frozen in crystallization buffer supplemented with 16-20% (v/v) glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 295K |