3I6W
Structure and Activation Mechanism of the CHK2 DNA-Damage Checkpoint Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Detector technology | CCD |
| Collection date | 2006-01-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.200, 114.700, 123.000 |
| Unit cell angles | 84.10, 81.20, 80.70 |
Refinement procedure
| Resolution | 30.000 - 3.250 |
| R-factor | 0.252 |
| Rwork | 0.251 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i6u |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.138 |
| Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.250 |
| Number of reflections | 60983 |
| Completeness [%] | 96.2 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1:1 ratio mix of protein solution (~12 mg/ml protein in 20 mM Tris-HCl, 150 mM NaCl, 10 mM dithiothreitol (DTT), 3% (v/v) glycerol, pH 8.0 and well bufffer (100 mM NaHepes, 300 mM Ammonium Tartrate, 22% PEG 3350, pH 7.0). Crystals were flash frozen in crystallization buffer supplemented with 16-20% (v/v) glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 295K |
