3I6G
Newly identified epitope Mn2 from SARS-CoV M protein complexed withHLA-A*0201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5478 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.871, 48.847, 137.242 |
| Unit cell angles | 90.00, 110.94, 90.00 |
Refinement procedure
| Resolution | 27.254 - 2.201 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zf1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.801 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.300 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 | 0.319 |
| Number of reflections | 50983 | |
| <I/σ(I)> | 31.682 | 5.051 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1M Ammonium acetate, 0.1M BIS-TRIS, pH6.0, 13-17% Polyethylene glycol 10,000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
