3I55
Co-crystal structure of Mycalamide A Bound to the Large Ribosomal Subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-29 |
Detector | ADSC Q315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 211.324, 299.649, 574.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.980 - 3.110 |
Rwork | 0.210 |
R-free | 0.26000 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
Rmerge | 0.171 | 0.061 | 0.927 |
Number of reflections | 323283 | ||
<I/σ(I)> | 6.2 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 7.5 | 7.3 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 292K |