3I55

Co-crystal structure of Mycalamide A Bound to the Large Ribosomal Subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-29
Detector	ADSC Q315
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	211.324, 299.649, 574.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.980 - 3.110
Rwork	0.210
R-free	0.26000
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.171	0.061	0.927
Number of reflections	323283
<I/σ(I)>	6.2
Completeness [%]	100.0	99.8	100
Redundancy	7.5	7.3	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	292	PEG6000, KCL, NH4CL, MGCl2, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	5.8	292	PEG6000, KCL, NH4CL, MGCl2, pH 5.80, VAPOR DIFFUSION, SITTING DROP, temperature 292K