3I4Z
Crystal structure of the dimethylallyl tryptophan synthase FgaPT2 from Aspergillus fumigatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2008-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9395 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.060, 98.790, 125.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.710 - 1.760 |
R-factor | 0.152 |
Rwork | 0.151 |
R-free | 0.18300 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.447 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.710 | 1.810 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.098 | 0.470 |
Number of reflections | 98932 | |
<I/σ(I)> | 14.14 | 2.2 |
Completeness [%] | 98.7 | 91.3 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 26% 1,3-butanediol, 50mM sodium L-lactate, 100mM sodium MOPSO pH 7.0, 2mM DTT, vapour diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 26% 1,3-butanediol, 50mM sodium L-lactate, 100mM sodium MOPSO pH 7.0, 2mM DTT, vapour diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP |