3I4U
Crystal Structure Analysis of a helicase associated domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.91164 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 78.854, 78.854, 88.793 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.480 - 2.100 |
R-factor | 0.19532 |
Rwork | 0.193 |
R-free | 0.24486 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.881 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Total number of observations | 557688 |
Number of reflections | 31239 |
<I/σ(I)> | 29.2 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2uL drop (1:1); 1.7M (NH4)2SO4, 0.1M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2uL drop (1:1); 1.7M (NH4)2SO4, 0.1M Tris/HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |