3I2B
The crystal structure of human 6 Pyruvoyl Tetrahydrobiopterin Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.290, 118.720, 234.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.940 - 2.300 |
| R-factor | 0.20841 |
| Rwork | 0.206 |
| R-free | 0.24566 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gtq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.483 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.940 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.792 |
| Number of reflections | 91910 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20% PEG 3350, 0.20M Mg(form)2, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
