3I1F
Gamma-subunit of the translation initiation factor 2 from S. solfataricus in complex with Gpp(CH2)p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 203.3 |
Detector technology | CCD |
Collection date | 2007-06-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8148 |
Spacegroup name | P 31 |
Unit cell lengths | 95.770, 95.770, 165.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.400 - 2.500 |
R-factor | 0.2281 |
Rwork | 0.226 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pmd |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.109 | 0.531 |
Number of reflections | 57747 | |
<I/σ(I)> | 7.73 | 2.2 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 2.83 | 2.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 15% natrium malonate dihydrate, 50mM glycine, 3mM CdCl2 at the protein/nucleotide molar ratio 1/10. For cryoprotection ethylene glycol was added to the reservoir solution at the final concentration of 15% (v/v), pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |