3I0W
Crystal structure of Clostridium acetobutylicum 8-oxoguanine glycosylase/lyase in complex with dsDNA containing cytosine opposite to 8-oxoG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-09 |
| Detector | MAR345 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.400, 92.400, 191.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 1.730 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f0z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.800 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.056 | 0.325 |
| Number of reflections | 48375 | |
| <I/σ(I)> | 32.2 | 4.93 |
| Completeness [%] | 94.7 | 79.2 |
| Redundancy | 14.2 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | PEG-4000 16%, 0.1M NaAcetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
