3HYC

Crystal structure of E. coli phosphatase YrbI, with Mg, tetragonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97869
Spacegroup name	P 41 21 2
Unit cell lengths	122.219, 122.219, 202.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.080 - 3.060
R-factor	0.24239
Rwork	0.242
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r8e
RMSD bond length	0.006
RMSD bond angle	0.980
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.080
High resolution limit [Å]	3.060
Number of reflections	29776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM Bis-Tris propane pH 6.0, 100 mM NaCl, 13.5 % PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K