3HXI

Crystal structure of Schistosome eIF4E complexed with m7GpppG and 4E-BP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-01
Detector	NOIR-1
Wavelength(s)	1.5
Spacegroup name	P 21 21 2
Unit cell lengths	45.311, 125.330, 37.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 1.800
R-factor	0.22355
Rwork	0.218
R-free	0.27158
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v8w
RMSD bond length	0.011
RMSD bond angle	1.348
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.000
High resolution limit [Å]	1.800
Number of reflections	22977
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100mM pH 6.5 Mops, 20% PEG4K, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K