3HXG

Crystal structure of Schistsome eIF4E complexed with m7GpppA and 4E-BP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-01
Detector	NOIR-1
Wavelength(s)	1.5
Spacegroup name	P 21 21 2
Unit cell lengths	45.560, 125.400, 37.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 2.100
R-factor	0.2155
Rwork	0.210
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v8w
RMSD bond length	0.009
RMSD bond angle	1.222
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.080
High resolution limit [Å]	2.100
Rmerge	0.113
Redundancy	3.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	279	100mM pH 6.5 Mops, 20% PEG4K, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 279K