3HV7
Human p38 MAP Kinase in Complex with RL38
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-06 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.54170 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.710, 74.520, 78.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.870 - 2.400 |
R-factor | 0.208 |
Rwork | 0.203 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.620 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Phasing software | PHASER (1.3.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.069 | 0.357 |
Number of reflections | 15927 | 3910 |
<I/σ(I)> | 17.1 | 5.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |