3HTU
Crystal structure of the human VPS25-VPS20 subcomplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.562, 51.043, 76.987 |
Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.24035 |
Rwork | 0.239 |
R-free | 0.27261 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cuo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.288 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 30553 | |
<I/σ(I)> | 29.1 | 3.03 |
Completeness [%] | 98.4 | 87.6 |
Redundancy | 3.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris-HCl pH 8.0, 35% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |