3HTM
Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPBTB/3-box
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Collection date | 2005-04-20 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.800, 88.700, 88.700 |
| Unit cell angles | 90.80, 89.30, 89.90 |
Refinement procedure
| Resolution | 44.340 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Number of reflections | 37547 | |
| <I/σ(I)> | 25.5 | 4.9 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
