3HTM
Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPBTB/3-box
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Collection date | 2005-04-20 |
Spacegroup name | P 1 |
Unit cell lengths | 36.800, 88.700, 88.700 |
Unit cell angles | 90.80, 89.30, 89.90 |
Refinement procedure
Resolution | 44.340 - 2.500 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.25600 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Number of reflections | 37547 | |
<I/σ(I)> | 25.5 | 4.9 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |