3HSY

High resolution structure of a dimeric GluR2 N-terminal domain (NTD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95294
Spacegroup name	P 21 21 21
Unit cell lengths	82.452, 93.695, 101.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.533 - 1.750
R-factor	0.178
Rwork	0.178
R-free	0.20500
RMSD bond length	0.006
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.840	1.840
High resolution limit [Å]	1.750	1.750
Number of reflections	79848
<I/σ(I)>		2.8
Completeness [%]	100.0	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	200mM ammonium phosphatre, 20% PEG3350, pH4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K