3HQD
Human kinesin Eg5 motor domain in complex with AMPPNP and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-09 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.500, 71.435, 94.846 |
| Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.190 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1X88 molecule A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.608 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM2 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 2.290 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.119 | 0.548 |
| Number of reflections | 41542 | |
| <I/σ(I)> | 9.35 | 2.27 |
| Completeness [%] | 99.2 | 94.1 |
| Redundancy | 2.85 | 1.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 40 mM KH2PO4, 20% glycerol w/v, 15% PEG 8k w/v, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
