3HPX
Crystal structure of Mycobacterium tuberculosis LeuA active site domain 1-425 (truncation mutant delta:426-644)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.025, 70.886, 70.043 |
| Unit cell angles | 62.45, 81.41, 70.26 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.17716 |
| Rwork | 0.174 |
| R-free | 0.23794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sr9 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.666 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.126 | 0.489 |
| Number of reflections | 47066 | |
| <I/σ(I)> | 17.7 | 3.8 |
| Completeness [%] | 94.5 | 84.7 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 291 | Sodium citrate, PEG MME 2000, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
