3HPV
Crystal Structure Analysis of the 2,3-dioxygenase LapB from Pseudomonas sp. KL28
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.23983 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.900, 97.000, 133.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.710 - 2.300 |
Rwork | 0.201 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mpy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.611 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.119 | 0.652 |
Number of reflections | 46741 | |
<I/σ(I)> | 23.294 | 3.6 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 13 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M ammonium fluoride, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |