3HOC

Structure of the actin-binding domain of human filamin A mutant E254K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2006-11-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.657, 73.937, 154.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.620 - 2.300
R-factor	0.22402
Rwork	0.222
R-free	0.26335
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wku
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.640	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.088	0.530
Total number of observations	122444
Number of reflections	29480
<I/σ(I)>	12.3	2.4
Completeness [%]	98.0	93
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K