3HOC
Structure of the actin-binding domain of human filamin A mutant E254K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.657, 73.937, 154.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.620 - 2.300 |
| R-factor | 0.22402 |
| Rwork | 0.222 |
| R-free | 0.26335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wku |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.640 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.530 |
| Total number of observations | 122444 | |
| Number of reflections | 29480 | |
| <I/σ(I)> | 12.3 | 2.4 |
| Completeness [%] | 98.0 | 93 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, 5mM DTT, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
