3HOB
Factor VIII Trp2313-His2315 segment is involved in membrane binding as shown by crystal structure of complex between factor VIII C2 domain and an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-02 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.04 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.279, 58.988, 60.086 |
Unit cell angles | 90.00, 110.65, 90.00 |
Refinement procedure
Resolution | 33.390 - 2.070 |
R-factor | 0.21966 |
Rwork | 0.217 |
R-free | 0.26278 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d7p |
RMSD bond length | 0.012 |
RMSD bond angle | 1.454 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 | 0.415 |
Number of reflections | 17899 | |
<I/σ(I)> | 13.492 | |
Completeness [%] | 93.1 | 63.9 |
Redundancy | 3.1 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 30.0% PEG 2000, MME 0.15M KBr, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |