3HN3

Human beta-glucuronidase at 1.7 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-06-19
Detector	Bruker AXIOM 200
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	93.578, 123.115, 266.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 1.700
R-factor	0.205
Rwork	0.203
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.264
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.730
High resolution limit [Å]	1.670	3.590	1.670
Rmerge	0.103	0.068	0.488
Number of reflections	316019
<I/σ(I)>	11.182
Completeness [%]	88.4	99.9	73.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10mM Tris-HCl pH 7.5, 15% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K