3HMX
Crystal structure of ustekinumab FAB/IL-12 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Collection date | 2002-06-04 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.092, 116.435, 182.781 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.660 - 3.000 |
R-factor | 0.215 |
Rwork | 0.211 |
R-free | 0.30300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.883 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.700 | 45.700 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.128 | 0.073 | 0.494 |
Number of reflections | 24337 | ||
<I/σ(I)> | 24.5 | 4 | |
Completeness [%] | 99.9 | 100 | 100 |
Redundancy | 13.22 | 12.57 | 14.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |