3HKS

Crystal structure of eukaryotic translation initiation factor eIF-5A2 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-06
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.560, 79.830, 94.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.920 - 2.300
R-factor	0.20435
Rwork	0.202
R-free	0.24074
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x6o
RMSD bond length	0.011
RMSD bond angle	1.266
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.920	2.280
High resolution limit [Å]	2.230	2.230
Rmerge	0.066
Number of reflections	20393
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	25% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K