3HK0
Crystal Structure of the RA and PH Domains of Grb10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.875, 48.884, 92.217 |
| Unit cell angles | 90.00, 114.14, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.23221 |
| Rwork | 0.230 |
| R-free | 0.27689 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.667 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.062 | 0.323 |
| Number of reflections | 16167 | |
| <I/σ(I)> | 13.8 | 3 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 310 | crystals were grown at 17 C in hanging drops containing equal volumes of protein solution and reservoir buffer (~15% PEG 3350, 0.15 M sodium thiocyanate, and 1 mM dithiothreitol (DTT)), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
