3HK0
Crystal Structure of the RA and PH Domains of Grb10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9798 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.875, 48.884, 92.217 |
Unit cell angles | 90.00, 114.14, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.23221 |
Rwork | 0.230 |
R-free | 0.27689 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.059 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.667 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.062 | 0.323 |
Number of reflections | 16167 | |
<I/σ(I)> | 13.8 | 3 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 310 | crystals were grown at 17 C in hanging drops containing equal volumes of protein solution and reservoir buffer (~15% PEG 3350, 0.15 M sodium thiocyanate, and 1 mM dithiothreitol (DTT)), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |