3HJW

Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-12
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	186.013, 63.026, 85.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.520 - 2.350
R-factor	0.218
Rwork	0.217
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hvy
RMSD bond length	0.010
RMSD bond angle	1.397
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.079	0.041	0.592
Number of reflections	51141
<I/σ(I)>	20.968
Completeness [%]	91.3	99.5	51.1
Redundancy	6.1	7.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	0.8 M KCl, 0.15 M Mg Acetate, 8% (w/v) PEG 6000, pH 6.5, vapor diffusion, hanging drop, temperature 303K
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	0.8 M KCl, 0.15 M Mg Acetate, 8% (w/v) PEG 6000, pH 6.5, vapor diffusion, hanging drop, temperature 303K