3HI9

The x-ray crystal structure of the first RNA recognition motif (RRM1) of the AU-rich element (ARE) binding protein HuR at 2.0 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00778
Spacegroup name	P 31 2 1
Unit cell lengths	74.804, 74.804, 147.361
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.760 - 2.000
R-factor	0.199
Rwork	0.197
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fxl
RMSD bond length	0.013
RMSD bond angle	1.361
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.000	2.000
Rmerge	0.096	0.484
Number of reflections	32767	3709
<I/σ(I)>	7.9	3.2
Completeness [%]	99.3	99.5
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2 M Di-ammonium citrate, 20% w/v PEG 3350, 5% Glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K