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3HI7

Crystal structure of human diamine oxidase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron siteAustralian Synchrotron
BeamlineMX1
Temperature [K]100
Detector technologyCCD
Collection date2009-01-30
DetectorADSC QUANTUM 210r
Wavelength(s)0.95663
Spacegroup nameP 21 21 21
Unit cell lengths92.506, 94.784, 196.525
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.570 - 1.799
R-factor0.16205
Rwork0.161
R-free0.18890
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)The starting model for molecular replacement was a partially refined model of the human diamine oxidase we had previously solved in C2221 this was solved using pdb code 2C10 as a starting model.
RMSD bond length0.011
RMSD bond angle1.345
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.860
High resolution limit [Å]1.7991.799
Rmerge0.0840.436
Number of reflections158683
<I/σ(I)>20.0343.7
Completeness [%]99.095.1
Redundancy6.45.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52980.1M bis-tris propane, 20%(w/v) PEG 3350, 0.2M sodium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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