3HHW
Complex of a vesicular stomatitis virus empty capsid with the nucleocapsid-binding domain of the phosphoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.94 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 170.602, 234.520, 95.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.26447 |
Rwork | 0.263 |
R-free | 0.29573 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qvj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.073 | 0.419 |
Number of reflections | 77814 | |
<I/σ(I)> | 20.79 | |
Completeness [%] | 73.1 | 44.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.8 M K/Na tartrate, 0.2 M NaCl, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |