3HE3

Crystal Structure of UDP-galactopyranose mutase in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9797
Spacegroup name	P 21 21 21
Unit cell lengths	134.050, 176.874, 222.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.880 - 2.400
R-factor	0.2108
Rwork	0.208
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v0j
RMSD bond length	0.004
RMSD bond angle	0.838
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.880	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.182	0.598
Number of reflections	204114
<I/σ(I)>	6.6	1.5
Completeness [%]	98.9	93.1
Redundancy		3.49

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	295	0.1 M HEPES, 0.2 M LiCl and 28% PEG 6000, pH 6.5, Microbatch, temperature 295K