3HDL
Crystal Structure of Highly Glycosylated Peroxidase from Royal Palm Tree
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.42 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 117.821, 117.821, 93.447 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.779 - 1.850 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.946 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.780 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 64039 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES pH 6.5 and 2.6 M ammonium sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
