3HCT
Crystal structure of TRAF6 in complex with Ubc13 in the P1 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-14 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.985, 42.798, 49.118 |
| Unit cell angles | 104.61, 99.59, 108.60 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2GMI 3hcs |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 4.430 | 2.060 |
| Rmerge | 0.037 | 0.034 | 0.065 |
| Number of reflections | 16146 | ||
| <I/σ(I)> | 39.692 | ||
| Completeness [%] | 91.7 | 74.8 | 91 |
| Redundancy | 1.9 | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 8% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
