3HCS
Crystal structure of the N-terminal domain of TRAF6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-29 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.28220 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.663, 80.757, 50.723 |
Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27100 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.074 | 0.485 |
Number of reflections | 26445 | |
<I/σ(I)> | 28.703 | |
Completeness [%] | 98.0 | 81.7 |
Redundancy | 6.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.19-1.33M Ammonium sulfate, 0.1M CHES pH 9.4-9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |