3HCS
Crystal structure of the N-terminal domain of TRAF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-29 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.28220 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.663, 80.757, 50.723 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.074 | 0.485 |
| Number of reflections | 26445 | |
| <I/σ(I)> | 28.703 | |
| Completeness [%] | 98.0 | 81.7 |
| Redundancy | 6.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.19-1.33M Ammonium sulfate, 0.1M CHES pH 9.4-9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
