3HBW
Crystal Structure of Human Fibroblast Growth Factor Homologous Factor 2A (FHF2A), also referred to as Fibroblast Growth Factor 13A (FGF13A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.218, 57.998, 52.236 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| Rwork | 0.219 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 23482 | |
| <I/σ(I)> | 64.5 | 13.7 |
| Completeness [%] | 94.8 | 86.8 |
| Redundancy | 8.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 22% PEG 4000, 0.2M ammonium sulfate, 0.1M HEPES-NaOH pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
