3HB1
Crystal structure of ed-eya2 complexed with Alf3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1. |
Spacegroup name | I 4 |
Unit cell lengths | 183.941, 183.941, 119.976 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.510 |
R-factor | 0.205 |
Rwork | 0.200 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3geb |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.590 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmerge | 0.065 | |
Number of reflections | 68962 | |
<I/σ(I)> | 35.5 | 5.5 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 2.4M NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |