3HAM
Structure of the gentamicin-APH(2")-IIa complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.700, 58.800, 81.400 |
Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.500 |
R-factor | 0.21319 |
Rwork | 0.210 |
R-free | 0.27103 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.301 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.600 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.042 |
Number of reflections | 25799 |
<I/σ(I)> | 21.4 |
Completeness [%] | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | unbuffered 16% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |