3H9E
Crystal structure of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) complex with NAD and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.960, 71.780, 81.260 |
| Unit cell angles | 90.00, 122.90, 90.00 |
Refinement procedure
| Resolution | 68.230 - 1.720 |
| R-factor | 0.1439 |
| Rwork | 0.143 |
| R-free | 0.17573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vyv CHAIN D |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.230 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.077 | 0.514 |
| Number of reflections | 72227 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 99.2 | 96.3 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 20% PEG 3350; 0.2M Na/K phosphate; 10% Ethylene glycol (protein buffer 10mM Tris, pH 8.5; 0.1M NaCl), VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
