3H90
Structural basis for the autoregulation of the zinc transporter YiiP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.657, 130.704, 115.757 |
Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
Rwork | 0.261 |
R-free | 0.27677 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.450 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.059 | 0.424 |
Number of reflections | 65270 | |
<I/σ(I)> | 18.6 | 1.4 |
Completeness [%] | 92.7 | 54.3 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | see reference, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |