3H87
Rv0301 Rv0300 Toxin Antitoxin Complex from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.557, 85.557, 155.611 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.520 - 1.490 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.17400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.486 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.050 | 0.497 |
| Number of reflections | 94698 | |
| <I/σ(I)> | 36.9 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 200mM Potassium Acetate, 7.5% PEG-3350, 50mM Tris, 500mM NaCl, 140mM Imidazole, 10mM TCEP, beta-mercaptoethanol, pH 7.0, microbatch, under oil, temperature 298K |
