3H5K
Crystal structure of the ribosome inactivating protein PDL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.011, 34.735, 120.630 |
| Unit cell angles | 90.00, 127.99, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.450 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REMO |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.038 | 0.030 |
| Number of reflections | 86690 | |
| <I/σ(I)> | 26.9 | 2.9 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 5.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.16M sodium acetate trihydrate, 0.1M sodium cacodylate trihydrate, 24%(w/v) polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
