3H5K
Crystal structure of the ribosome inactivating protein PDL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-10-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.011, 34.735, 120.630 |
Unit cell angles | 90.00, 127.99, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.450 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REMO |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.510 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.038 | 0.030 |
Number of reflections | 86690 | |
<I/σ(I)> | 26.9 | 2.9 |
Completeness [%] | 99.9 | 98.4 |
Redundancy | 5.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.16M sodium acetate trihydrate, 0.1M sodium cacodylate trihydrate, 24%(w/v) polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |