3H4X
Structure of a Ca+2 dependent Phosphatidylinositol-specific phospholipase C (PI-PLC) Enzyme from Streptomyces antibioticus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.82653 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.968, 154.819, 41.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.970 - 1.230 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.988 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (CCP4_3.2.25) |
Refinement software | REFMAC (refmac_5.5.0068) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.990 | 1.300 |
High resolution limit [Å] | 1.230 | 1.230 |
Rmerge | 0.049 | 0.260 |
Total number of observations | 100339 | |
Number of reflections | 97776 | |
<I/σ(I)> | 8.2141 | 3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.28 | 7.11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 21% PEG 10000, 85mM sodium acetate pH 4.6, 170mM ammonium acetate, 9% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |