3H3O
Glycerol Kinase H232R with Ethylene Glycol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2008-02-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.844, 104.275, 114.242 |
Unit cell angles | 90.00, 114.13, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.20600 |
Structure solution method | PHASER |
Starting model (for MR) | 1xup |
RMSD bond length | 0.006 |
RMSD bond angle | 1.327 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.500 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 92379 | |
<I/σ(I)> | 11.1 | 2.1 |
Completeness [%] | 98.5 | 97 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | KH2PO4, PEG 8000, VAPOR DIFFUSION, temperature 298K |