3H2V
Human raver1 RRM1 domain in complex with human vinculin tail domain Vt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-19 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 41.818, 70.921, 99.342 |
Unit cell angles | 89.77, 90.04, 104.04 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.2131 |
Rwork | 0.210 |
R-free | 0.27690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rke |
RMSD bond length | 0.011 |
RMSD bond angle | 1.203 |
Refinement software | BUSTER-TNT (2.3.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 23190 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100 mM Tris (pH 7.5), 200 mM sodium nitrate, and 16% PEG-3,350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |