3H2U

Human raver1 RRM1, RRM2, and RRM3 domains in complex with human vinculin tail domain Vt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-19
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	86.754, 94.906, 186.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.750
R-factor	0.227
Rwork	0.225
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rke
RMSD bond length	0.011
RMSD bond angle	1.335
Refinement software	BUSTER-TNT (1.3.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.750
Number of reflections	40684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	100 mM Tris (pH 7.5), 200 mM sodium nitrate, and 16% PEG-3,350, VAPOR DIFFUSION, SITTING DROP, temperature 295K