3H1W
Crystal structure of mannose 6-phosphate isomerase bound with zinc and yttrium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.980, 92.360, 111.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.950 - 1.940 |
| R-factor | 0.18439 |
| Rwork | 0.182 |
| R-free | 0.23649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pmi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.202 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.061 | 0.290 |
| Number of reflections | 27871 | |
| <I/σ(I)> | 18.9 | 4.2 |
| Completeness [%] | 96.5 | 83.3 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Micro-bactch under oil | 6.5 | 293 | 0.1 M magnesium acetate, 0.2 M sodium cacodylate pH 6.5, 20% PEG 8000, 5% dioxane, 10mM zinc chloride, 10mM yttrium chloride, Micro-bactch under oil, temperature 293K |
