3H0F
Crystal structure of the human Fyn SH3 R96W mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2004-01-27 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.542 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 52.103, 52.103, 53.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.300 - 2.610 |
R-factor | 0.20912 |
Rwork | 0.202 |
R-free | 0.28226 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shf |
RMSD bond length | 0.017 |
RMSD bond angle | 1.703 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.300 | 2.720 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.150 | 0.630 |
Number of reflections | 2270 | |
<I/σ(I)> | 13.4 | 1.7 |
Completeness [%] | 91.9 | 58.2 |
Redundancy | 5.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 277 | 2.0M ammonium sulphate, 2% PEG 400, 0.1M Tris, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |