3GW6
Intramolecular Chaperone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-15 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 62.650, 79.950, 109.580 |
Unit cell angles | 81.71, 76.53, 87.11 |
Refinement procedure
Resolution | 27.633 - 2.600 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.25450 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.727 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.042 | 0.411 |
Number of reflections | 184838 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 95.2 | 95.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15 % PEG 8000, 0.1 M BisTris pH 6.0, 0.15 M CaBr2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |