3GVW

Single-chain UROD F217Y (YF) mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	103.291, 103.291, 71.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.810 - 2.800
R-factor	0.202
Rwork	0.194
R-free	0.27400
RMSD bond length	0.016
RMSD bond angle	1.739
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.086	0.034	0.704
Number of reflections	11174
<I/σ(I)>	19.754
Completeness [%]	100.0	99.7	100
Redundancy	7.5	6.9	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop			hanging drop