3GVP
Human SAHH-like domain of human adenosylhomocysteinase 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-01 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 164.188, 164.188, 184.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 2.250 |
R-factor | 0.18231 |
Rwork | 0.182 |
R-free | 0.22581 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d64 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.513 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.910 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.104 | 0.507 |
Number of reflections | 119253 | |
<I/σ(I)> | 33.2 | 9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 29.6 | 29.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES, 12.5% PEG3350, 0.2M sodium malonate, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |